Enumeration of enterotoxigenic Bacteroides fragilis in municipal sewage
نویسندگان
چکیده
منابع مشابه
Detection of enterotoxigenic Bacteroides fragilis by PCR.
Strains of enterotoxigenic Bacteroides fragilis (ETBF) are associated with diarrhea in young farm animals and, at least in particular settings, in children. Enterotoxin production by ETBF is currently detected by a tissue culture assay with HT-29 cells. We have developed a PCR assay based on the detection of the enterotoxin gene to identify ETBF in culture and in stool samples. Overall, 113 bac...
متن کاملAssociation of enterotoxigenic Bacteroides fragilis with bacteremia.
Polymerase chain reaction (PCR) assay was compared with cell culture assay performed with use of HT29/C1 (human colonic epithelial) cells for identifying strains of enterotoxin-producing Bacteroides fragilis (ETBF) isolated from extraintestinal specimens. A total of 188 unselected strains obtained over 2 years at a central clinical laboratory in Tokyo were tested. Overall, 35 strains (18.6%) we...
متن کاملEnterotoxigenic Bacteroides fragilis: a rogue among symbiotes.
Enterotoxigenic Bacteroides fragilis (ETBF) strains are strains of B. fragilis that secrete a 20-kDa heat-labile zinc-dependent metalloprotease toxin termed the B. fragilis toxin (BFT). BFT is the only recognized virulence factor specific for ETBF. ETBF strains are associated with inflammatory diarrheal disease in children older than 1 year of age and in adults; limited data suggest an associat...
متن کاملCulture and decontamination methods affecting enumeration of phages infecting Bacteroides fragilis in sewage.
A new medium has been adapted for the growth of Bacteroides fragilis so that its phages can be recovered from environmental samples, and its efficiency has been assessed. Polyvinylidene difluoride membranes allow significantly higher recoveries among different membrane filters used to decontaminate the samples. In all cases, a number of phages remain in the filters and a percentage of them can ...
متن کاملRapid detection of enterotoxigenic Bacteroides fragilis in diarrhoeal faecal samples.
BACKGROUND & OBJECTIVES Enterotoxigenic Bacteroides fragilis (ETBF) is an important aetiological agent of acute diarrhoeal illness in humans. It is a fastidious anaerobe and takes 3-5 days when grown in culture, and test for enterotoxin detection requires additional time conventionally. The present study was aimed to standardize a rapid and sensitive diagnostic assay and to screen the presence ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1990
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.56.7.2243-2244.1990